How can I test the tolerance of my bacteria to bile salts and pancreatic enzymes? What is the simplest method to do that? Thanks!

20 October 2014 1 8K Report

I need some detailed explanation concerning how to sterilize bile salts and pancreatic enzymes through bacterial filter paper?

Alaa Kareem Niamah

you can use bile salt from OX add 1.5 % W:V in MRS and autoclaving at 115 for 15 min .after Bacteria growth on this media , It's Bile salt tolerance

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