I need a method to show that a mammalian membrane transporter such as zinc transporter has its activity after cloning and expression in yeast host cells. Is there any way to test the activity?
Hello
You can read this link , Hope help you
Good Luck
https://www.hindawi.com/journals/ijpg/2009/296482/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709882/
Hello Bahram,
Read this article to get an idea.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709882/
Hello,
you could use a yeast mutant altered for Zn transport (eg. zrt1∆), which does not grow in zinc-limited medium, and check whether the expression of the mammalian transporter in these cells rescues growth on this medium.
See https://www.ncbi.nlm.nih.gov/pubmed/8637895
Beware that the heterologous expression of transporters in yeast cells can prove difficult. See https://www.ncbi.nlm.nih.gov/pubmed/16897711
Good luck
Sebastien
Dear Sebastien
Thank you very much for your helping, of course it is possible, however creating mutation is itself another project. I am seeking to find an easy approach.
sincerely
pooreydy
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