I need to study the anti-inflammatory activity of the crude Mtanolic extracts .
What is the method?
USE FOLLOWING METHOD https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609167/ Anti-inflammatory activity of the leaf extacts of Gendarussa vulgarisNees
Hi
you can collected blood and after centrifuge, measure the parameters such as TNF-alpha and IL-1beta by kit using.
I hope these links will help you even more;
http://www.sciencedirect.com/science/article/pii/037887419490085X
http://www.sciencedirect.com/science/article/pii/S0024320501014825
http://www.sciencedirect.com/science/article/pii/S0308814611010107
http://www.sciencedirect.com/science/article/pii/S0378874108005643
https://www.ncbi.nlm.nih.gov/pubmed/20728594
Please read the paper intitled" Measurement of inflammation and evaluation of anti-inflammatory agents" indexed in PubMed database.
anti-inflammatory activity can be evaluated using (Human Red Blood Cell) HRBC membrane stabilizing method.
See the attached article
https://www.ncbi.nlm.nih.gov/pubmed/27814587
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