How can I study the ability of phosphorous solubilisation in bacteria without using PVK agar medium?

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What is the date of birth of your loved one?

Your Bro

23 April 2024 251 1 View

MATLAB code for the Centroid and APIT localization algorithms (wsn)?

Hi, I need the MATLAB code for the Centroid and APIT localization algorithms (wsn) to verify the results I obtained with the DVHOP algorithm. Thank you in advance. Best regards.

05 February 2024 6,119 4 View

How to fix the defect after optimized the geometry of a modified ZIF..?

Hello everyone, I am a beginner in computational studies. Little familiar with Material Studio. I modified the ZIF-8 structure with an ethyl group. After modification, when the geometry was...

29 January 2024 1,145 0 View

How can I calculate the sample size for a study aiming to evaluate the practice of community pharmacist?

The estimated sample size was 280 according to the population (registered pharmacist). Since I am going to use two diffrent prescriptions to evaluate their practice, will the sample be 280×2 or...

29 December 2022 5,055 6 View

How can I increase RNA yield from RNA-IP?

I did an RNA-IP out of cells and my RNA yield for the inputs was great but the yield was poor for the IP samples. Is there a way to increase the yield of RNA? (other than increasing the starting...

27 November 2022 2,847 8 View

How to determine the local Nusselt number along a pipe in Ansys?

I'm trying to detect the local Nu number at different planes. My issue is that when I open the variable option in chart, I can't get the "surface Nusselt number" to show up under the variables...

31 January 2022 7,327 2 View

Small RNA ladder on bioanalyzer problem?

Hello, I have a problem with the detection of the small RNA ladder on the bioAnalyzer for the quantification of small RNAs. I contacted Agilent, they sent me another ladder. I had the same...

05 January 2022 6,215 0 View

Sample program for simulating photodetectors?

Hello Dear rima I want a software model to simulate photodetectors by the simulation software Silvaco means the most important models in it, especially if the photodiode is from a Schottky...

24 October 2021 8,555 3 View

How to choose GC-MS and HPLC analysis conditions?

Dear all, I have to realize GC-MS and HPLC for my bacterial extract (the solvent used was ethyl acetate). I would like to know how could I choose the experimental conditions for that (injection...

18 April 2021 1,163 6 View

Can anyone suggest me a model or tools to apply in doing verbal language in trailers?

I am using visual perspective but the study needs the verbal aspect as well to be analysed for more comprehensive research. Thank you in advance

18 March 2021 1,130 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,755 4 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Why do we use Horse Serum for C2C12 cell differentitation?

I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...

04 July 2024 4,732 1 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Why don't my intestinal organoids from children's polyps grow?

Hi! I am having trouble growing intestinal organoids from children's polyps. Sometimes they don't grow at all, and other times they look dark and unhealthy, as shown in the attached pictures...

18 June 2024 4,781 3 View