I've been working on the development of rapid tests using whole blood, but I'm having difficulty to create a barrier in the red cells and separating just the plasma; the nitrocellulose membrane gets a little bloody. I've already treated the sample pad with 5% PVP on PBS1X, it reduced the blood flow, but it's still not ideal. I wouldn't want to use a membrane to separate the plasma. I would like to do a sample pad treatment. can anybody help me? Thanks
Have you tried doing the reverse, treating the membrane with PBS?
Could test different concentrations of PBS... (maybe at that concentration of PBS, the size of the blood cells decreases a lot... allowing them the flow through the membrane)...
If i repeated something you already know, i'm sorry
Dharliton Soares Gomes thanks, but I would like to have a specific treatment to stop of the bood cells in the sample pad, because I need only plasm in contact with conjugate pad.
Prakash Rana Thanks. It´s too expensive. I think that there is a specific treatment to sample pad, but I don´t have a recipe.
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