Error while running the cif2cell script?

I am getting this error every time I run the cif2cell script, How can I solve the problem? $ cif2cell -f graphenerelaxed.cif -p quantum-espresso Traceback (most recent call last): File...

13 July 2020 6,425 1 View

How to install ShengBTE in ubuntu?

I am trying to install this software but it doesn't work

12 June 2020 6,352 1 View

Will it be sufficient to calculate properties of a material (in quantum espresso) using the unit cell only?

Will it be sufficient to calculate the properties of a material with the atoms in the unit cell only, like in the attached, or do I have to make a super-cell? what difference does it have?

22 May 2020 7,087 13 View

How can I calculated transport properties of layered materials across plane and in-plane?

I want to calculate the cross-plane and in-plane transport properties of layered materials using quantum espresso and boltztrap codes. I don't understand how to choose between in-plane and...

15 October 2019 5,573 2 View

How much does bead beating for cell lysis heat the samples (reg. pre-cooled adapters for the Qiagen Tissue Lyser II) ?

Hello, I would like to isolate total protein for phosphoproteomics using the Qiagen Tissue Lyser II (that we have in our department) for disruption of yeast lysis with glass beads. However,...

10 October 2019 8,861 3 View

How to prevent reoxygenation of cells after incubation in hypoxic chanbers?

We are using hypoxia chambers to induce physiological hypoxia in cell cultural environment. the major problem is, reoxygenation during the cell harvesting, cell lysis, etc. this is the chamber we...

08 June 2016 1,646 4 View

How to do the COMET ASSAY for cell line?

Complete PROTOCOL needed

18 April 2016 7,269 3 View

Does anybody know any hepatocellular carcinoma cancer cell line control drug for treatment?

27 September 2015 2,148 9 View

Is anyone familiar with performing MTT assay for Plant extracts?

MTT assay concentration fixing stock solution perpetration and require volume for test.

04 March 2015 150 2 View

Can any one suggest Protocol for isolation of proteins from cancer cell lines?

protein isolation

05 February 2015 4,886 3 View

No title

02 March 2021 3,060 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View