I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end all that remains are very small debris of gel or nothing at all.
Arnaud Martino Capuzzo
Hello Ayman Abdelrahim I suggest you look at these three links:
1)
Article Bioink formulations to ameliorate bioprinting-induced loss o...
2)
3) Article Review of 3D printable hydrogels and constructs
Article Cell Damage Evaluation of Thermal Inkjet Printed Chinese Ham...
Let us know if you've solved it, good job! If you need anything else write it down.
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