How does a nafion membrane work to block interference?

In bio-sensor applications nafion is often used to prevent anionic interfering species (e.g ascorbate, urate etc) from reaching the electrode surface while letting the sensor sensitive to only...

04 May 2016 7,034 2 View

Solvent for Cellulose Acetate?

04 May 2016 5,174 10 View

How can I fabricate a Ag/AgCl reference electrode?

06 July 2015 1,016 16 View

Is there anyone who worked with MLX90129?

How does MLX90129 work as a transponder chip or RFID tag? It does not even have its own antenna. We can actually fabricate our own RFID tag simply just having an antenna along with a capacitor....

05 June 2015 6,227 1 View

How to Chloridize silver nanowires?

I am working on biosensor using silver nanowires. Does anyone have any idea how to chloridize silver nanowires for reference electrode?

04 May 2015 3,062 3 View

What are the reactions going on in Ag/AgCl reference electrode?

Hi, Ag/AgCl reference electrode uses an electrolyte of KCl solution saturated with AgCl. The most often seen reaction in literature is AgCl= Ag++ Cl-. But what are the actual reactions going on...

31 December 2014 5,983 4 View

Tutorials CHI 1200 series Electrochemical analyzer?

Is there anyone who has used or have any idea on this series? I badly need some tutorials specially on CV analysis. Any suggestions/ links would be much appreciated.

31 December 2014 8,939 3 View

How to test standard potential of Ag/AgCl reference electrode?

Hi, I am trying to fabricate my own Ag/AgCl reference electrode. How can I measure the potential of it? What is the process people usually do to check the potential and the stability of potential...

31 December 2014 5,708 10 View

Why is Ag/AgCl used as a reference electrode in amperometry?

Most of the amperometric experiments done I have seen in different publications used Ag/AgCl as reference electrode. Why is it necessary to do? What is the advantage of using Ag/AgCl electrode...

11 December 2014 5,205 3 View

How do you use an Ag/AgCl reference electrode for amperometry?

Is it necessary to have the Ag wire dipped into a KCl solution during using it as a reference electrode? Or can I just use the Ag wire having a layer of Chloride on it?

11 December 2014 4,477 8 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View