The Nusselt correlations for the high aspect ratio and high Rayleigh number?

I want to calculate natural convection in the horizontal rectangular enclosure (heated from below) and in the vertical rectangular enclosure by using the Nusselt correlation. However, I can not...

25 April 2020 648 9 View

qRT-PCR melting curves are good for cDNA of overexpression cells but not for cDNA of parental cells. What could be the reason?

I am trying to find a good qRT-PCR primer set for a gene that has low expression in our cell lines. As you see in the attachment I have a very sharp one peak when I used cDNA from overexpression...

23 February 2020 2,474 5 View

Viscosty effects in 1H NMR measurements?

I would like to see the derivatization of my polymer by 1H NMR. However, I cant see any peak. My sample becomes viscous in water (in the case of NMR it is in D2O). I did a similar test before and...

22 August 2019 5,020 3 View

What kind of skills, different from ordinary entrepreneurs, innovative entrepreneurs must have in terms of successful marketing?

With the new technological era, ordinary entrepreneurs leave their places to innovative entrepreneurs and the old way of marketing has also started to change. Innovative entrepreneurs must have...

02 March 2018 1,908 8 View

Anyone encountered this "Interesting" signal in LC/MS runs yet: 321.16 m/z?

It is (1+) and strongly present with high ACN content at the end of our LC gradients (Setup: Ultimate 3000RSLCnano HPLC coupled to an impactII MS running plant proteomics samples). Didn't find any...

09 March 2016 5,155 6 View

How to fix unstable GUVs with particles/tubes inside?

After electroformation, our unilamellar vesicles act very dynamic and unstable on BSA passivated slides (especially as their sizes get bigger). We sometimes observe them bursting and reforming....

07 February 2016 2,858 4 View

Could CGRP antagonists be helpful in the fight against COVID-19?

Can CGRP antagonists, which are seen as a new treatment option in migraine treatment, be a treatment option in COVID-19. What is your opinion? I share a study on this

01 January 1970 9,487 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View