Dear Sir,

In answering your question I need to know (a) if you have a dry (powder) mix or a mixed suspension or solution and (b) if starch granules can still be observed by microscope.

Kind regards, Peter A.M. Steeneken

Peter A. M. Steeneken

Dear Peter,

Thank you for answering.

(a) It is more to suspension

(b) I didnt check it with microscope. However, I have gelatinized the starch.

This is how the i prepare it. I dissolved starch into certain amount of water and heated it to 80 degree Celsius. After that, I dissolved SSG in cold water and then pour it into the starch solution to mix it. I carried out some synthesize process afterward. After that, i would like to separate both of them.

Best Regards,

Chua

Hello Siong Chin Chua

when you added two compound with same polarity and solubility for example starch and gums , so there will be many of interactions between the functional heads of two compounds for example hydrogen bond.

thus if you want to separate two compound with same polarity you should break their interactions ! and after that you could separate them by their difference in molecular weights and centrifuge.

i think you could ask your question in this way that "How can i break interaction between two material with same polarity?"

good luck

Bahman nazari

In case you have a dry mix of SSG and ungelatinized starch granules the answer is simple: because SSG is soluble in cold water and intact starch granules are not, just add excess water to obtain a low viscosity aqueous suspension. SSG will dissolve and the starch granules will settle. Starch is separated by (repeated) sedimentation. SSG can be recovered from aqueous solution by ethanol precipitation. [Microscopic examination (optionally with iodine staining) of your sample prior tho this experiment reveals whether the starch is in the granular state.]

Hello Bahman Nazari ,

That exactly the question i want to ask next. Haha.

Btw, Thank you so much for answering.

Hello Peter A. M. Steeneken ,

Thank you for the answer provided, appreciated. In case both of them, SSG and gelelatinized starch mix in water, any way to separate it ? Thanks

If the starch is gelatinized the matter becomes more complicated. However, if you only want to recover SSG but are not interested in the starch the simplest approach is to enzymatically degrade the gelatinized starch with alpha-amylase and/or amyloglucosidase and then remove the malto-oligosaccharides by dialysis. SSG can then be recovered by ethanol precipitation.