How can I separate large size plasmid (200kb) from gram positive bacteria bacillus anthracis? .

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Basil A. Abbas

by different centrifugation

Jorge Barros-Velázquez

Hi Vikas,

as Basil suggests, ultracentrifugation in CsCl gradient will provide you with the two different bands, genomic and plasmid DNAs. You can check the protocol in the classic Molecular Cloning textbook by Sambrook et al. (1989)

best regards