How can i separate 3,5-Dimethylphenol and it's positonal isomer by using reverse phase hplc?

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Guobing Xiang

Maybe regular silica gel column will be better? Did you take the silica gel TLC? I do not think 3,5-Dimethylphenol and it's positonal isomer will dissolve in water well if you use reverse phase HPLC.

Sandeep Chandrashekharappa

What is the positional isomer of 3,5-Dimethylphenol ?

Rouvain Bension

Did you try the conditions in the manufacturers' brochures? When you open the page, search for "dimethylphenol"

https://www.separatedbyexperience.com/documents/ANCCSCETISOMER_0611.pdf

https://www.agilent.com/cs/library/applications/A01449.pdf

http://phx.phenomenex.com/lib/5732_I.pdf