Can anyone suggest a lab or university, preferably in Australia that can test for the isomer ratios of a commercial paclobutrazol product?
Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...
25 June 2024 5,799 0 View
There is research into the models individually, but not one seeking to find an overall best one suited for any condition.
23 June 2024 5,641 3 View
I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...
12 June 2024 4,027 1 View
Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...
10 June 2024 9,942 5 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,928 0 View
I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...
28 February 2024 213 2 View
Hello All, I have several questions about qPCR. I will explain what I have done to give you a clear picture. I have 2 kidney samples. 1 wildtype and 1 KO for a specific gene. We have extracted...
25 February 2024 6,331 2 View
I have extracted genomic DNA from 2 tissues. 1 wild type and 1 KO. I havd designed primers that should amplify a region in between the two loxp sites. I want to validate the effectiveness of the...
22 February 2024 9,660 0 View
I have raw qPCR data. 2 samples "1 control and 1 with gene knock out". I made a serial dilution of each sample and performed a qPCR using 2 reference genes and 3 genes of interest. Now I have the...
21 February 2024 2,745 5 View
Hi! Does anyone have ideas to separate Fe(II) complexes from Fe(III) complexes? Context about the complexes: 1) Coordination of the ligand causes some type of Fe(III) to Fe(II) reduction...
07 February 2024 8,464 2 View
I am working on some isomerization processes. Please help me how I can use RRKM methods. which software can be used to find the rate constants.
18 December 2023 8,906 0 View
Dear All, I would like you to help me solve a problem in Gaussian. The intention is to evaluate the influence of temperature on the cis/trans isomerization process for a certain compound. Once...
20 August 2023 5,953 0 View
Through pyridine infrared, we observed that the zeolite contained Lewis acid, and through 27Al NMR, we also observed the existence of six-coordinated Al species in the sample. However, by...
15 February 2023 5,575 0 View
Hi All, I encountered the following problem when trying to perform NBO calculation with Gaussian 16 software for the cluster Ce3O7AgCu in the gas phase: the calculation was terminated with the...
26 December 2022 3,361 0 View
That is A has fully converted to B
20 September 2022 1,324 2 View
I am looking for a software/online tool that will help to identify possible sites prone to post-translational modifications (including deamidation, isomerization, protease cleavage, oxidation...
10 May 2022 9,206 2 View
Actually my synthesized macrocyclic ligand has three different isomer. Now when I want to draw these in pdb format then no option is found to mention axial or equatorial group. If I don’t mention...
26 April 2022 2,675 0 View
Which API should be chosen for formulation development of Sotorasib tablets- Racemic mixture (mixture of enantiomer) or single isomer? Does therapeutic efficacy depend on enantiomeric form of...
09 February 2022 8,650 3 View
we need the reaction kinetics for light naphtha isomerization when catalyst pt base on chlorinated gamma alumina is used.please guide us, in which book or article we can find it.
23 October 2021 7,547 3 View
Eg: Cmax of Levocetirizine will be equal to Cmax of Cetirizine or half ?
19 September 2021 6,184 1 View