Is there a function in Gromacs for quantify ion bridge?

Hi all, I had simulate charged polymers and surfaces, and is very common to have interaction of ion bridge (or ionic crosslinking) which consist of and interaction of two equally charged groups...

24 June 2020 1,678 1 View

What is the common method for the analysis/identification of phytohormone-like compounds ?

Hi fellow researchers, Currently i'm doing some research in fungi-secreted phytohormone-like compounds (possible bioactive ones), and i want to know which method (for example, HPLC or GC-MS)...

06 March 2020 9,946 3 View

No title

25 September 2019 1,812 1 View

How to obtain surface charge density from experimental data?

Greetings, I need the surface charge density of particulate solid at diferent pH. I have tried from the surface complexation model but there are several things missing. I only have the zeta...

27 September 2018 3,616 2 View

I need to prepare a solution of hesperidin for spectrophotometric readings, which solvent do you recommend?

I am having troubles in disolving hesperidin, I tried Dimethylformamide and acetic acid 0.01 M (20:80) but it is not working.

30 October 2017 6,883 2 View

Which is the best antibody to label the cilium?

03 September 2016 9,554 3 View

Is it possible to use an OPLS potential in conjunction with other potentials originally created with an arithmetic mixing rule for sigma?

I'm using Gromacs 5.0.2 for a MD simulation with polymer in a saline solution, but the parameters for the polymers are not know, so I'm using OPLS, but this create a incompatibility with the other...

26 April 2016 966 3 View

Can anyone help me with this chromatogram?

It is a chromatogram of phenolics compounds (standard compounds) and with a red arrow I point the place where a high peak (Peak 1) appears when I inject a methanolic extract from black mulberry...

17 March 2016 9,973 2 View

Is anyone familiar with a scale to measure attitudes or beliefs on high impact environmental projects (for example, dams)?

02 April 2015 4,120 7 View

How to control CO2 levels during live cell imaging?

Our microscope-integrated incubator is not working properly. Can we add some carbon tabs to the culture wells to control the CO2 level during imaging? Where can we obtain those tablets? Thanks

11 November 2014 9,925 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

What effect does the large temperature difference between the two primers have on PCR, including real time PCR?

What effect does the large temperature difference between the two primers have on PCR, including real time PCR? If I did not get a result from real time PCR, is it possible that my problems are...

21 February 2021 4,094 7 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View