I build a chamber (10ul) in a PDMS for PCR application but when I heat the chamber (after 5-6 cycles), evaporation occurred and vanish the liquid in the chamber.
It seems the inlet and outlet of the chamber have leakage.
Is it possible that the vapor leak from above of the chamber (2mm PDMS)?
Hi,
Thanks Jaroslav,
No,I don't check it. It is possible to have leakage from PDMS. I should check it. but at first I should completely seal the inlet and outlet. I don't know how to do it.
We completely closed the inlet and outlet then we filled the chamber. after about 30 min 40% of solution evaporates from PDMS (3mm layer) !! Is any method to solve this problem? or we should change the PDMS with other material like PMMA, Glass,..?
Dear Mohammad reza,
welcome,
So, it seems that the PMDS is permeable to the vapor the material enclosed in it.
May a remedy is to seal the surface of the chamber by coating it with thin film of noble metal like gold.
Best wishes
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