I am getting a band in negative control as well and so I want to check whether the other bands I am getting are positives or false positives.
If the band in the no template control is the same size as the bands in the positive samples then you have pcr amplimer contamination in a reagent,pipette or working area.. You cannot know whether the bands in the sample tubes are real amplification from the samples until youclean up your contamination problem. Only then can you be confidant about the sample amplifications
What you have is contamination. You can't trust any of your data.
Toss ALL of your reagents (primers, water, buffer, taq, etc.). Make them again from fresh, never been used before stocks. Pro tip: have one micropipette designated for loading gels. Never use the same micropipette for handling amplified DNA that you use to set up PCR.
Good luck!
Based on your activity here on RG and previous few questions, it seems you are overly relying on help via RG, instead of trouble-shooting yourself or asking for proper help and guidance from your colleagues. This is not a good sign and it can harm your research ability, critical thinking and trouble-shooting abilities. Use this platform and a last option when you have tried all other options and nothing works.
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