Dear Jayachandran, it might be that alkalinization by - say 0.5M NaOH - may be able to destain your sections from Rose Bengal.
BUT: I am not quite sure about since
i) I haven't had a necessity to destain sections as you want to do, and, ii) I haven't found for now - without a thorough search of the specific literature - any special or specific hint on such a method.
But with the reported changing of amino groups' properties = ionisation (NB: for intensified staining with Rose bengal one adds acetic acid, for diminished staining one has to increase the pH, which last but not least / in the end (completely) inhibits the attachment of the dye) it could be possible that already stained sections lose their dye-molecules. Perhaps application of the usual alkalinization solutions = e. g. blueing agent(s), either dilute ammonia or saturated lithium carbonate are sufficient for a successful destaining of your sections.
If this is (would be) a real possibility to follow (one should test that), for other / further / repeated staining one should incubate sections long enough in (acetic) acid solution (one drop to 0.5 ml/1 L washing water).
Regards and best wishes,
Wolfgang
Look at this info: http://stainsfile.info/StainsFile/dyes/45440.htm
Rose bengal should act in the same manner as eosin. And Eosin is washed out of the tissue by water and diluted ethanols rather easily.
If dyes are rather sensitive for destaining sometimes it is not even necessary to destain slides, that one wants to use for additional applications (like IHC).
Dear Jayachandran, just to add: naturally one can try also only "water" and / or "diluted ethanols" as Gudrun says. BUT: might depend also on the task you deal with ("remove Rosebengal stain from sample"). Therefore guessing you might have stained not (tissue) sections but e. g. macro- or micro-benthic marine "samples" instead I would like to point you to a paper discussing depending on state of sampling and (first) staining:
DONNAY et al, 2013 [in: Medit. Mar. Sci., 14/1, 2013, 92-94] stating:
".....When the staining was done immediately before sorting, the destaining consisted of one bath with 95% ethanol. When staining was completed some hours before use, the destaining consisted of two baths using alkaline solution. In the latter case, each bath was 30 min in duration: the first one with only alkaline solution (NB: in general alkaline solution(s) [pH 9.0] - not mentioned which one exactly! - according to THIEL et al, 1966), the second with the alkaline solution and 95% ethanol in a 1:1 mix. Finally, in all cases, organisms were stored in 95% ethanol. ....."
It would be interesting to read about the "samples" you stain(ed) and the outcome of your trials on destaining.
Best regards and good luck,
Wolfgang
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