How can I remove the pigments from the dry plant before the phytochemicals extraction because the color of the pigments does not make me see the zone of inhibition in the antimicrobial bioassay?
try disc diffusion method or simply dilute sample in ug/ml for better result.
I diluted the sample but no i did not get result only in 100mg/ml concentration.
Thank you so much Ankit Negi sir i will try disc diffusion method as you suggested me in high concentration to get result
Pigments are phytochemicals...
You can try and pre-extract (wash) your sample with solvents that will extract the pigments but not your constitute.
Either way 100mg/ml is a very high concentration to begin with. What are you extracting with?
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