I am working on isolation, purification and characterization of Quercetine from leaf extracts. I have acetone extract of leaf and I want to remove the chlorophyll from it first. i tried n-Hexane separation but it haven't worked
Hello!
Dissolve the extract in ethanol, then add hot water dropwise shaking (approximately 60 degrees). Ethanol and water in a 1: 1 ratio. Leave overnight at room temperature. After a day, a precipitate falls. Lipophilic compounds precipitate.
Acetone is exclusively used to isolate Chlorophyll, so if possible use other solvent for isolating your compound. If you need to isolate by Acetone only, then you can try to degrade the chlorophyll content by heating your extract, because at high temperature chlorophyll get degraded. Just make sure that the compounds of your interest don't get degraded on heating.
Read the method of (Jarrett et al. 2004) ISOLATION AND PURIFICATION OF QUERCETIN
2,3-DIOXYGENASE FROM ASPERGILLUS FLAVUS VIA
LECTIN AFFINITY CHROMATOGRAPHY. Journal of Undergraduate Chemistry Research
You can use charcoal to absorb the color of chlorophyll, then purification with filter paper.
Hello!
Dissolve the extract in ethanol, then add hot water dropwise shaking (approximately 60 degrees). Ethanol and water in a 1: 1 ratio. Leave overnight at room temperature. After a day, a precipitate falls. Lipophilic compounds precipitate.
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