Dear all,
I am having a technical problem with our FACS Canto II. It seemed somehow the device picked up some air bubbles and now it does not complete the fluidics start up neither the purge. I tried doing SIT flush several time and degas the flow cell. Both were completed successfully yet still the device kept giving the red alarm that fluidics start up can not be completed. I tried then Bubble filter purge several times yet it failed all the time. Any recommendation or tipps how to remove air bubbles from the system?
Thanks in advance
I think, reducing the surface tension will do the trick. If the system permits, add ethanol to e.g. 20% or add a detergent like Tween 20 to 0.5% m/v.
If the problem persists, your system needs cleaning.
Wolfgang Schechinger I did already the long cleaning for the system. adding ethanol wouldn't it affect the system?
Usually, 20% EtOH is fine for systems which are handling biological fluids. The machine's manual should have a list of compatible reagents. If in doubt, contact their tech service.
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