How can I remove biogenic nanoparticles protein capping? Does anybody have a methodology ?

More Mariana Guilger Casagrande's questions See All

Can anyone help me with microplates reading?

I performed assays in microplates for the first time to determine enzymatic activity, but I made a mistake with the reading. The recommended wavelenght for the assay is 550 nm. I used an ELISA...

04 May 2019 2,701 13 View

I am looking for a methodology to remove protein capping from silver nanoparticles synthesized through mycogenic route. Can anyone help me?

I am looking for a methodology to remove protein capping from silver nanoparticles synthesized through mycogenic route. I already tried a methodology, but because of centrifugation steps I lost...

09 October 2017 3,237 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

Muhammad Ismail Popular answer

You want to remove the surface capping agents like amino acids????

Nora Hilda Paños

Hi Mariana,

Why do you consider the silver nanoparticles are biogenic? Which is the biomass containing or producing the silver nanoparticles?

Mokhtar Nosrati

Hi,

If the nanoparticles are coated with certain proteins you can use SDS-PAGE for separating.

Muhammad Ismail