09 September 2014 3 1K Report

I try to extract keratin from human hair and make 3D scaffolds for cell culture. I use what is called as Shindai method for extraction (b-mercatpoethanol, urea, thio-urea buffered in Tris-Cl). After extraction, I dialyze the extract against water for 4 days.

I am not able to grow cells on the scaffolds and I figured out there was b-mercaptanol remaining in the extract. Could tell me how can I remove b-ME completely.

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