How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Dear Colleagues, I am looking for a way to dop of electrospining nanofibers on the HEPA filter.Someone can guide me?

Electrospining method is important for production of nanofiber .If doped with nanoparticles ,can be used for remove of contaminant such as VOC , but don't have mechanical strengh . For finding a...

24 January 2021 7,290 3 View

What is the current situation in the development of nanofibers laminated and biodegradable material based facemasks?

I want to know the progress in the development of polymer laminated, biodegradable material based facemasks.

18 January 2021 9,205 3 View

What could be the appropriate load and loading rate to carried out nanoindentation for testing microhardness of nanofiber?

Dear Reader, Recently, I have prepared a polymeric nanofiber using PVA polymer doped with some nanoparticles and I want to find out its microhardness. So, I was planning to do...

09 January 2021 4,452 7 View

How can I take an electrical conductivity test from a nanofibrous scaffold?

I have a nanofiber scaffold for tissue engineering. When I did the electrical conductivity test, the ohmmeter reports a wide range of values (from 300 kOhm to 20 MOhm). I placed the scaffold...

06 January 2021 2,232 1 View

DSC test- Glass transition temperature of the electrospun PA66 nanofibers?

Hello Everyone, I did several DSC tests on the electrospun PA66 nanofibers to obtain the glass transition temperature. According to the material datasheet, the Tg point of PA66, as cast, is around...

13 December 2020 1,506 2 View

Which are the best methods to graft the polymeric nano/micro particles with lipids?

Good references (with clearly written methods/protocols) are highly appreciated. Polymers could be any, e.g., PLA, PCL, PLGA, etc. Similarly, lipids could be DSPE, DMPE, DMPA, DPPE, DLPC, DOTAP,...

07 December 2020 5,085 4 View

How do I avoid sucking up electrospun scaffolds during cell culture?

Hi, I'm a PhD student working to develop electoconductive, electrospun scaffolds for cardiac tissue engineering applications. I have been seeding cells onto these scaffolds but having major...

04 December 2020 6,672 3 View

Does crystallinity degree, (and crystalline phase) of a polymer affect its surface energy and adhesion?

I have several PLLA electrospun nanofibers with different crystallinity degrees and also different crystalline phases and I am measuring the adhesion force by AFM-based techniques. I wonder if we...

28 November 2020 3,269 5 View

Looking for non-toxic super absorbent and hydrogels with low flexural strength?

I am looking for a non-toxic and ineffective hydrogel that can be converted into fibers by electrospinning. I also need to know its solvent. Since I am going to use these fibers for medical...

14 October 2020 9,936 8 View