Hi everyone, I got a C-rich linear strand DNA and want it to form i-motif upon low pH. The question is how can I prove the formation of the i-motif in solution. Has anyone done that? Maybe CD or electrophoresis?
Circular dichroic (CD) spectroscopy
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665218/
Hi,
Circular Dichroism is a good tool, if i-motif forming you should get a positive peak at around 288 nm and a negative peak at around 260 nm. The paper the poster above gave you is very good. If you don't have CD available, another option is UV using thermal difference spectroscopy.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201377/
Finally another option is NMR, if i-motif is forming it will give very characteristic proton peaks at around 15.5 ppm which represent the immino protons on the protonated N3 of cytosine.
Cheers
Henry
Circular Dichroism is a good tool, if i-motif forming you should get a positive peak at around 288 nm and a negative peak at around 260 nm. The paper the poster above gave you is very good. If you don't have CD available, another option is UV using thermal difference spectroscopy.
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