4 Questions 3 Answers 0 Followers
Questions related from Guangqi Wu
Hi there, I'm doing western blot recently. My SDS-PAGE gel was first stained with the Coomassie Blue G250 and then after disstaining I transferred it to a PVDF membrane. After the transmembrane...
02 February 2017 3,336 2 View
Hi everyone, I got a C-rich linear strand DNA and want it to form i-motif upon low pH. The question is how can I prove the formation of the i-motif in solution. Has anyone done that? Maybe CD or...
07 July 2016 1,394 3 View
Hi everyone, I want to hybridize a 30nt-long poly dT on mRNA (which have polyA sequence). I extracted the total RNA and let it hybridize with a 5‘ FITC labelled polyT30 (heat to 95℃ for 5 minutes...
07 July 2016 968 2 View
Dear all, Currently I am using DNA synthesizer (ÄKTA oligopilot plus) to synthesize oligonucleotide(20-50nt), whether phosphodiester and phosphothiolated. However, I've been stucked in the...
03 March 2016 8,792 3 View
