What is the best protocol used for this purpose? Would you suggest a few papers please
You could preserve the tissue in paraffin (FFPE) blocks which can be kept for ages (use microtome to cut slides for IHC and histopathology) or preserve fresh tumour tissue in OCT media (but then you need to keep them frozen) and have access to a cryotome if wanting to cut sections for histopathology or immunohistochemisty like your question tags suggest.
In case you want additionally to isolate nucleic acids or protein in future (after ages), you could also consider reagents such as RNAlater or Allprotect Tissue Reagent for the preservation and keep the samples frozen.
This is an age old method for preservation but it really works do have a look. Its called the Amex method.
This depends on what you are planning to do with the tissue and how much tissue you have got available.
My preference would always be to fix some tissue in formalin and get it processed into paraffin blocks. This allows good assessment of morphology even after decades (provided you store the blocks adequately in a dry environment), Also, you can now do most immunohistochemical and molecular analyses on paraffin-embedded tissue. Nevertheless, if you have got the facilities and sufficient tissue material to start with, it might be a good idea to snap-freeze an unfixed tissue sample and store it at -80°C.
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