How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

Which kind of GPC solvent can be used to dissolve both lipid and salt?

I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).

14 January 2021 3,805 2 View

How to see the expression of 6kda protein?

Dear all, I am now investigating the expression of small size protein (6 kda). However, the expression of the protein cannot be shown clearly after performing western blot. Therefore, are there...

13 January 2021 3,121 3 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

Bell's inequality from probability theory?

It is said Bell's inequality is a consequence of probability theory, which has nothing to do with quantum or not quantum. There are many papers discuss this issue, but I don't know which one is...

22 December 2020 8,254 3 View

Do I need to add a start codon and stop codon for the sgRNA expression in crispr?

Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...

16 December 2020 5,795 3 View

Could you please interpret the data and SAXS patterns of lipid nanoparticles for us?

Dear Prof. Weiss, Recently, we prepared lipid nanoparticles (composed of phophatidylserine and aluminum ions), and we characterized the particles using SAXS. However, we are unable to interpret...

15 December 2020 4,292 3 View

Are there any method to detect implanted stem cells?

Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...

15 December 2020 6,065 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View