Considering that you have a stock standard of high concentration (for example 10000 ug/ml), it would be helpfull to prepare a mid-concentration solution, such as 1000 ug/L. Then with a simple dilution you can achieve the desired concentrations. The important is in every dilution, the dilution factor not exceed the value of 1000.
Thanks a lot. But the problem is how to prepare stock in ug/mi as chloroform is in liquid state?
You can dissolve the liquid standard into an adequate organic solvent, such as ethanol. Chloroform is not soluble in water but is soluble in mosto of organic solvents.
As always: take the pure compound, put some (drops) of it in a meassuring flask; determine with a balance how much You have exactly. Fill up with organic solvent. As chloroform is volatile You need to work
a) quick b) in a cold environment c) in meassuring flask that You can close gas tight.
dilute with the solvent of choice.
solvent of choice depends a bit on what You want to do with it afterwards. Fo GC determination I guess it would be wise to use a solvent that is even more volatile
Hi,
You may use the web tool to calculate
https://www.alfa-chemistry.com/solution-dilution-calculator.htm
https://www.alfa-chemistry.com/mass-molarity-calculator.htm
I remember that chloroform has the tendency to attach to glass when in high concentrations in water, like good wine. Is that true?
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