Hi every body
responce of my HPLC decreased.
I think , UV cell detector is dirty.
How can I I Wash UV cell detector?
I washed Whole HPLC with Water, water+methanol and methanol.
Are there any way to wash?
firstly make sure the problem not from the column , by the way you can make the routine wash for the whole system by the following sequence :
1-start with 100 % water(hplc grade) for 15 min with acceptable flow rate
2- 80:20 (water : ACN or methanol ) for 15 min
3- 50:50 (water : ACN or methanol ) for 15 min
4_100% (ACN or methanol ) for 15 min
if the problem not solved (reverse the uv conections and flush system with isopropanol for 5 min )
Dear Sharif.
Thanks for your answer
I did the routine wash, but I have extra peak and responce low.
Dear mahsa
Is there any leakage from the flow cell ?
second, try to replace the column ..what the result ?
Last , try to reverse the detector connections (outlet in the inlet ) then flush with isopropanol (HPLC grade) for about 5 min .. then reconnect the system in the correct direction ..
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