I need albumin unfolding
Could you recommend some articles on Urban Transportation System optimization and Innovation?
13 August 2024 2,595 3 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...
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Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Articles on" Gender disparities i leatherwork education"
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Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...
03 August 2024 4,787 2 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...
01 July 2024 2,124 2 View
Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?
28 June 2024 2,885 6 View
Fertilizers play a crucial role in enhancing the productivity of plants. However, low nutrient use efficiencies of conventional fertilizers (CFs) associated with several losses have led to...
21 June 2024 8,843 3 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...
16 June 2024 5,876 5 View
Urea fertilizer concentration percentage of nitrogen
07 June 2024 488 5 View
Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...
04 June 2024 1,501 0 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View
Need a simple and small scale preparation methods
23 May 2024 3,843 0 View
