How can I prepare Agrbacterium tumifasience containing transformation vector with targeted gene ?

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Kaushik Kumar Panigrahi

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC150518/

Hope this article will give you a preliminary idea.

https://www.researchgate.net/publication/236903222_Agrobacterium-mediated_transformation_of_potato_cv_Mnandi_for_resistance_to_the_potato_tuber_moth_Phthorimaea_operculella

Some more for your further reference

Article Agrobacterium-Mediated Plant Transformation: the Biology beh...

https://www.ncbi.nlm.nih.gov/books/NBK217998/

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/agrobacterium-tumefaciens

https://www.sciencedirect.com/science/article/pii/S0167701211003228

https://academic.oup.com/mmy/article/47/5/485/1049872

http://www.plantphysiol.org/content/129/1/13

https://www.nature.com/protocolexchange/protocols/427

https://www.nature.com/protocolexchange/system/uploads/1871/original/Protocol.pdf?1312300398

Muhammad Jakir Hossain

Thanks a lot, Dear Sir Kaushik Kumar Panigrahi for your kind help.