How can I prepare a recombinant rabies virus to trace its transport in live neuronal cells using confocal microscopy?

10 June 2015 1 2K Report

The recombinant rabies virus is used in transport mechanics within neuronal cells. I need to know the procedure to prepare the fluorescence tagged virus?

Andrew John Murray

Have a look at this paper, they label the rabies glycoprotein with dsRed and image transport of the virus in neuroblastoma cells

http://jvi.asm.org/content/82/1/237.long

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