I am working on frost tolerance in faba beans. I have two treatments (after and before frost). I measured the shoot fresh matter and dry matter in these two cases. The correlation between shoot fresh matter before and after frost was positive. Also, the correlation between shoot dry matter before and after frost was positive.
Do you have scientific interpretations supported from other literature?
Ahmed, high positive correlation between shoot fresh/dry matter before and after frost is expected from the plant growth. If you correlation is significant (r>0.7-0.75) It shows just the fact that the frost has not damaged your plants, or part of damaged plants was low.
If your correlation between dry mass and frost before and exposure to the stress is high (r > .85), you can argue that maintaining dry mass under frost stress is an indicator for frost resistance. In this case, for sure frost stress has minimal injury to your material.
Methods to determine plant freezing tolerance are either only semi-quantitative, such as scoring plants for survival after freezing and thawing, or do not allow high-throughput measurements, such as the electrolyte leakage assays (Ehlert and Hincha. 2008, Sergios and Howell, 1973; Murray et al., 1989). Electrolyte leakage is generally supposed to monitor primarily the intactness of the plasma membrane as a diffusion barrier (Ehlert and Hincha. 2008).
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