I want to do H&E staining of U-937 cells and for that I am looking different protocol and not able to find that how steps like washing can be done with non adherent cells. as it will be washed away in that procedure.
is there any alternate protocol of H&E for suspension cells?
I want to study morphological changes in U-937 cells due to treatment with my chemical(Drug) for that I want to perform this.
Good day, dear collegue. I may recommend to use one of the methods of cell block
http://ajcp.oxfordjournals.org/content/139/2/177
hi. in my laboratory we use the Nathan solution (formalin 37% (10%) + absolute alcohol (90%)) to fix and coagulate the cells in the fluid and then prepare cell block. i have good results.
greetings.
Article A Simple and Efficient Method for Preparing Cell Slides and ...
Refer this article. A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips for suspension cells
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