How can I perform an ex-vivo eye permeation study for the posterior segment disease?

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Vishnu Rompicharla

Dear Dignesh,

Ex-vivo permeation studies can be performed using goat eye corneal membrane. You don't need to fix the entire eye in the diffusion cell. Simulated tear fluid has to be used as medium for drug release and permeation. Make sure that the corneal membrane is in contact with the medium.

Good luck!!

http://www.hindawi.com/journals/isrn/2013/826798/

Dignesh Khunt

Thanks for given answer. But actual i want know that i am targeting posterior segment of eye, so just corneal membrane can give me idea about that my formulation is reach to the posterior segment of eye or not ?