What Will Be The Most Efficient Method To Perform Column Chromatography Of The Below Structured Compound?
And What Will Be The Stationary Phase
And The Mobile Phase Elute For This Compound?
Kindly Suggest?
I suggest you start off by finding perhaps three organic solvents such as alcohol hexane or toluene in which your dye may dissolve. The next step is to select a number of candidate absorbent materials such as cellulose (in the form of paper), or colourless polyester or acrylic fabric in either ground up form or fibrillary mass, thus enabling a thin-layer chromatographic analysis.
You next put dissolved dye spots on each candidate substrate followed by a steady flow of solvent drops, causing the component dye structures to separate out into a set of circular bands. You then assemble your column using the solvent/substrate combination with best success in clearly separating the components.
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