ferric iron induced oxidized LDL formation

Dear Anandita,

Usually, oxidizing metal ions (e.g. Fe or Cu) use for in Vitro oxidizing LDL. Briefly:

LDL (100 pg protein/ml) incubates with either 6 pM FeSO4 or 10 pM CuSO4 in tissue culture wells for up to 24 h at 37°C in triplicate at the indicated pH value. This gives a final concentration of EDTA of 5 microM and a net concentration of CuSO4 of 5 microM taking into account the EDTA concentration. EDTA-copper complexes are inactive as regards LDL oxidation in simple phosphate buffers but Low concentrations of EDTA do not inactivate FeSO4 in

 simple phosphate buffers. For details please see attached file.

http://www.jlr.org/content/36/12/2504.full.pdf

best

mehdi

Dear Anandita, 

I do not have personal experience in oxidizing LDL; however, I have used  vitamin C and FeSO4 combination quite effectively for platelet protein oxidation and it really gives very good result. I think as a lipoprotein it will also respond. In presence of Fe asocorbate behaves as strong oxidant-dehydroascorbate.

best wishes,  I will be happy if you convey that what worked best for you.

Dr. Kalyan Goswami

Thank you for your opinion Sir

Dear Anandita,

Please read our paper, wehre you will find what you need.

Best regards,

Günther 

Title: Binding and uptake of differently oxidized low density lipoprotein in mouse peritoneal macrophages and THP-1 macrophages: Involvement of negative charges as well as oxidation-specific epitopes

Author(s): Wang, XS; Greilberger, J; Ledinski, G; et al.

Source: Journal of Cellular Biochemistry Volume: 81 Issue: 3 Pages: 557-569 Published: 2001

CuSo4 works well

@Guenther Juergens

Dear Pr.,

I read the article you cited (3 years ago), thanks a lot. however there is something I don't get : what is the goal of nitrogen saturation, and how can it be performed, please ?

Best regards,

Richard