I try to crystallize miniprotein by hanging drop method. Here is a drop picture, where the precipitant is 1,4 Dioxane (35%). I think it looks like crystals but they does not have sharp edges and look like walnuts with round shape. Could you suggest me how can I optimize conditions and get more sharp and good crystals?
I think it is because of fast growth probably? Should I try seeding from these crystals?
Dear Svetlana Korban many thanks for your interesting technical question. As an inorganic chemist I'm not an expert in protein crystallization, but I can suggest to you some potentially useful references which might help you answering your question. Please have a look e.g. at the following articles entitled
1. Introduction to protein crystallization
Article Introduction to protein crystallization
This article is freely available as public full text on ResearchGate.
2. X-ray structure analysis of a designed oligomeric miniprotein reveals a discrete quaternary architecture
https://www.pnas.org/content/101/33/12183
(see attached pdf file)
@ Svetlana Korban
Could try seeded Crystal growth by maintaining the suitable temperature ( Water Bath)
Dear Svetlana,
Did you test these crystals on a diffractometer? Some of the crystals look single. In order to improve the crystallization, I would try additives. We had four times success with neutral additives that would detwin twinned crystals (see Article N,N,O, and N,O,N meridional cis coordination of two guanines...
, once with methanol and https://doi.org/10.1021/ic010790t).Good luck!
Best regards
Bernhard
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