Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Which is the importance of fixing (e.g. bivalvia) haemocytes and use anti-clotting solutions in flow cytometry?

I am trying to optimize a flow cytometry method to characterize mytilus spp. haemocytes. In literature I found that someone, to avoid clotting, use anti-clotting solutions or dilute and fix the...

05 November 2020 2,522 4 View

Has any of you encountered issues with protein production in a E.coli cell-free protein expression system?

I'm having trouble synthesizing a mollusk 173 aminoacids protein of unknown function in a E.coli cell-free protein expression system. The positive control works well and the plasmid has been...

01 October 2019 8,829 7 View

How I could set up a lipoplexe-based transfection protocol and compare data with commercial Viromer Red transfection reagent?

I am new in the field of transfection and I'm having a lot of problems in setting up the protocol with LIPOPLEXES in order to compare data previously obtained using Viromer Red protocol on THP-1...

13 November 2018 3,545 1 View

Does anybody know a reasonable (in prize) company where to buy an environmental chamber to adapt a confocal microscope for live cell imaging?

We are interested in adapting a Confocal microscope for long-term live imaging, and for this reason we are trying to get a small environmental chamber at a reasonable prize. It could be enough...

16 May 2017 5,437 2 View

How to store a purified protein before a kinase assay?

23 September 2013 8,697 1 View

Is it possible to position the HA taq not at the extreme end of a protein and visualize the taq-protein by Immunocytochemistry?

06 June 2013 5,942 9 View

What are the best antibody markers to detect and quantify the circulating endothelial cells and progenitors by flow cytometry?

CD45/CD31/CD34/KDR or CD45/CD31/CD146/CD133? Or both or neither?

18 February 2013 1,958 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View