Hello everyone,
I am looking for a nuclear marker, like a transcription factor, expressed in human enteric neurons.
I would like to characterize my human iPSC-derived culture with immunofluorescence stainings. The problem is that my neural progenitors give rise to enteric neurons and enteric glia (GFAP+), so I cannot use Sox10 throughout the whole maturation. So far I haven't found anything this specific.
Does any of you have any suggestions about a good antibody, or a marker that would be useful in this case?
Thanks a lot in advance.
Dear Elisabetta,
Please You look at the following article:
Article Derivation of enteric neuron lineages from human pluripotent...
The migratory cells that come as a result of embryoid body and subsequent neural rosette formations have been shown to be positive for neural crest specific markers Sox10, TFAP2A, BRN3A, ISL1 and ASCL1, and a subset found to be positive for regionally specific vagal markers HOXB2 and HOXB5, even without the inclusion of RA (23)
Enriched NC populations were then co-cultured with primary gut explants in a Transwell system to promote ENC identities enriched for HOXB2, HOXB3, HAND2 and EDNRB.
23,Characterization and transplantation of enteric neural crest cells from human induced pluripotent stem cells.
Li W, Huang L, Zeng J, Lin W, Li K, Sun J, Huang W, Chen J, Wang G, Ke Q, Duan J, Lai X, Chen R, Liu M, Liu Y, Wang T, Yang X, Chen Y, Xia H, Xiang AP
Mol Psychiatry. 2018 Mar; 23(3):499-508.
Article Characterization and transplantation of enteric neural crest...
Dear Alexandr,
Thank you for the answer. Unfortunately, I already know these works really well!
I can't use neural crest markers in IF as most of them are either cytoplasmic or downregulated before the enteric neurons become terminally differentiated.
Sox10 works quite well up to a certain point, but it's absent in mature neurons: its expression is maintained in enteric glia only.
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