I perform Xanthine oxidase enzyme reaction which will generate ROS. I want to neutralize the ROS (superoxide anion,H2O2). I only know that H2O2 could degraded by catalase.But i am wondering how to neutralize superoxide anion with some chemicals not SOD. could DTT degrade them?
Thanks.
You can try reduced glutathione (GSH) but upon reaction with ROS, it will also produce GS radical.
GS radical will get neutralize by formation of glutathione disulfide (GSSG).
The similar question has been already discussed
Can anyone suggest specific scavenger(s) for superoxide but not for ROS?
I am looking for the reagent which can scavenge superoxide in living cells. Most of ROS scavenger, like tempol, inhibit not only superoxide accumulation but also reactive nitrogen spices (RNS). But I am actually looking at the effect of RNS in oxidative stress but not superoxide. Dose anyone know the cell permeable superoxide scavenger?
Can anyone suggest specific scavenger(s) for superoxide but not for ROS? - ResearchGate. Available from: https://www.researchgate.net/post/Can_anyone_suggest_specific_scavengers_for_superoxide_but_not_for_ROS [accessed Dec 24, 2016].
I'm completely confused what Amit Kumar Mukherjee suggests. Dear Amit, please write the reaction you suggest to use.
Dear Amit,
the reactions that you wrote do not make any sense.
"2O2- + H2O = HO2- + OH- ;
2HO2- = 2OH- + O2.
(Find more like this as you wish-)"
They are just absolutely wrong.
"Also, superoxide acts as a very powerful oxidizing agent in water."
Absolutely wrong, superoxide is a mild reducing agent.
I downvoted your answer.
Dear Amit Kumar Mukherjee ，Yurii V Geletii and Ramesh K Jha
Thank you for your reply. In my experiment, I found 10 mM DTT could decrease superoxide anion cause I could not see the induction of OD value increase by redical detector but do observe uric acid production.
all my best,
Simeng
Dear Amit,
It’s unethical to disclose a private message to public forums. You don’t necessarily have to name just offer some means that any third party could identify who you are talking about. Therefore you forced me to disclose my message to you. Here it’s.
“Dear Amit,
I did not have any intention to hurt you and thus to show my superiority. I know this area in depth and therefore asked you for details. I did not agree with you and therefore commented your answer. I'm a reviewers of papers submitted for publication in numerous top journals and I used the similar wording to respond you as I do as a reviewer. I avoid any discussion in RG in the areas where I'm not an expert.
I agree that RG forum is far from perfect. The most of questions are trivial, while some of them are helpful to learn something in depth.
I upvoted your last comments. I'll appreciate much if you answer to my question
Yurii V Geletii asked a question in Scholar:
How to deal with ill-posed questions?”
Your way to discuss scientific problems is not acceptable for me. I do not downvote your last comments.
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