Hi Steev,

the bioanalyzer automatically change the scale of (s) to (nt) if it can right allign markers in the samples with markers to the ladder and/or if all the peaks in the ladder are correctly identified. You can check if in the ladder all the peaks are ok, if no you can try to give a manual integration.

Elena, I really apreciated your help. I had some problems with the ladder. The bioanalyzer just identified the first peak however, I do not know what happened with the other(s) peak(s). Also, I don not know what I did wrong, because before I load samples to the bioanalyzer, I load them into the nanodrop. It shows that they had a considerable concentration of RNA total. I am attaching a picture that shows that I explained to you.

One more thing, I am working with the Small RNA kit.

Hi Steev,

in your electropherogram I noted that the marker peak don’t appear in the samples and in the ladder you can see a 10 fold high peak (usualy 50 FU). If the quality of your samples is ok at the Nanodrop evaluation (A260/280 and A260/230) and the ladder and all the other reagent aren’t expired, I think that the problem may be connected with the chip preparation protocol. You have to verify that:

the base plate of the chip priming station is in position C;

the lever of the syringe clip is in the lowest position ;

whait exactly 60 seconds before relase the plunger;

visualy inspect that the plunger moves back at least to 0.3 ml mark.

I hope it helps

Elena

Hi Elena. Let me check what I did wrong. I think You are right. I forgot to check the priming station before I loaded the chip. I hope I can contact to you in a short period of time for telling what happen with my experiment. Best regards.

Steev Loyola

Hi Steev,

I am facing the same problem with High Sensitivity DNA Assay kit, can you please tell how did you resolve this issue?

thanks

Hey,

Did anyone figure out how to change it? I am also stuck on it