For example in a specific process If I use 1mg of silver in silver nitrate form the total mass of nanoparticles of silver would weigh how much ? Whether all silver ions have been reduced to silver atom or not?
Are you saying you have 1 mg of Ag or 1 mg of AgNO3? You cannot gain or lose the mass of silver in the (total) system.
Measure the concentration of silver ion by atomic adsorption before and after reduction. You will get the % reduction of Ag+ to AgNPs
Muhammad - be aware though that the surface of the Ag nanoparticles is Ag2O (that is silver in the +1 oxidation state) not silver metal. It's Ag+ that's the effective bactericide not Ag metal. See:
September 2nd, 2015 Silver colloids and invisible ink
https://www.brainshark.com/malvern/vu?pi=560497681&b=1&tx=120486&c1=22
AAS doesn't distinguish between Ag+ and Ag0
This is a common problem in nanoparticle yield calculation. The best method I wud say, first centrifuge your sol to get only Ag np (all unredused Ag should be decanted), then pour very high concentrated acid (raw HNO3 may be) to ensure complete oxidation of Ag and a stable low pH. Take the UV, and compare that with concentration calibration of known Ag in conc HNO3 to get Ag content in ur sample. Make sure to keep acid conc very high, because u would want pH of sample and calibrations to be similar and stable.
I recently measured the Ag concentration using ICP-AES after synthesis of AgNP using microalgae. I used NaCl solution to precipitate unreacted Ag+ as AgCl and then measured the stable AgNP in the supernatant. Here is the article (it has detailed method). ICP-MS would give you more accurate results though.
https://www.mdpi.com/1420-3049/24/1/98
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