How can I measure synaptic density in different brain regions using HIC and confocal microscopy?

04 November 2021 1 9K Report

I was thinking about VGlut2 (presynaptic) and PSD95 (post-synaptic) for the excitatory synapses, and VGAT (presynaptic) and Gephrin (post-synaptic) for the inhibitory ones.

Should I define as a synapse only the areas of colocalization between pre and post synaptic marker? Or should I consider also the isolate VGlut2/VGAT and PSD95/Gephrin ?

Qianfa Long

I think that you can use the Golgi staining to measure the density

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