I have prepared a Gelatin-chitosan-Bioglass composite 3D scaffold for bone tissue application.How do i prepare my sample for FTIR analysis as i found that grinding the scaffold was not that much beneficial.Is their any other process for preparing the sample?
Hi Kanchan
Try reflection (ATR) rather than transmission. With transmission you will get the bulk glass properties rather than the surface properties. In my opinion the surface information of the glass is of greater interest and from the studies I have performed on calcium aluminosilicates and bioglasses transmision and ATR give significantly different spectra. However, if you would prefer to use transmission try cryomilling your sample
Alternatively, you could also try mapping an area if you have a microscope on your FTIR have a look at this paper/Volume for sone informatio
S.G. Kazarian, K.L.A. Chan Applications of ATR-FTIR spectroscopic imaging to biomedical samples Pages 858-867 In Vibrational Microscopic Imaging: Towards Molecular Pathology. Biochimica et Biophysica Acta (BBA) – Biomembranes Edited by R. Mendelsohn and M. Diem Volume 1758, Issue 7, Pages 813-974 (July 2006)
I think i need to perform cryomilling for my sample.to perform this i need some reference for the cryomilling procedure, that is weather the sample should be fully immersed i liquid nitrogen , if so, then how long and how many times i should do this ??
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