I would like to work on atherosclerosis and obesity. I need an animal model for this work.
Look at the effects of nutrition on epigenetics and the methylation of the RNA particularly those of the 1 and 5 prime ends. There are not enough studies out yet to prove that indeed "we are what we eat"
There are quite a few models available. Is there a reason that you want. to create your own? Charles River Labs has an easy to use search engine for locating the right model. You can use C57Blk/6 mice in models of obesity, depending on what you are trying to do. There is some interesting work on the subject of nutrition and epigenetics, Look at the research of Sir Peter Gluckman if you are enticed by Dustin's suggestion. Fascinating, that.
Dear dustin, thanks for your suggestion. i will be think about my research on that way.
Dear oseid... thanks for your valuable information... actly my work to check the effect of herbal medicine(Biochemical aspects). so i need these nutritional/ chemical animal model for my work.
Most of the models I have worked with involve high fat feeding, so you may want to consider how high fat might interact with your herbal treatments. and dosing of your test compounds
You can use LDL receptor-deficient (LDLR-/-) mice fed to high-fat diet with 0.15% cholesterol (HFC), and a high-fat, high-sucrose diet with 0.15% cholesterol (HFSC). LDLR-/- mice fed HFSC or HFC developed significant diet-induced obesity, while HFSC-fed mice also developed atherosclerotic lesion area.
Piggy backing off of Magna, I would say that it is easily beneficial to also look at the effects of the all sugars, most particularly those found in the processed foods. Eg: fructose and its furthered effect on liver toxification.
u can use cholesterol, cholic acid (Cholic acid), and lard. it is given ad libitum at least for 14 till 21 days.
I recommend a guinea pig model because the serum lipid profile of guinea pigs and their pattern of lipoprotein metabolism is similar to that of humans. They are also highly sensitive to changes in the fatty acid composition of their diets. Feeding guinea pigs with with foods high in dietary saturated fats and cholesterol (like palm kernel oil) added to their grain-based chow for a period of 12 weeks will get you their. Am attaching some papers that will give you a comprehensive information.
Good luck.
From: John
Another file attachment - I discovered I can only attach one file at a time
I recommend a guinea pig model because the serum lipid profile of guinea pigs and their pattern of lipoprotein metabolism is similar to that of humans. They are also highly sensitive to changes in the fatty acid composition of their diets. Feeding guinea pigs with with foods high in dietary saturated fats and cholesterol (like palm kernel oil) added to their grain-based chow for a period of 12 weeks will get you their. Am attaching some papers that will give you a comprehensive information.
Good luck.
From: John
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