Hi everyone,
I hope to make an appropriate buffer for in vitro transcription. Could anyone help me with an easy-to-perform protocol to make a buffer for in vitro transcription in the laboratory?
Here is a protocol found on-line:
https://openwetware.org/wiki/Sauer:In_vitro_transcription_with_T7_RNA_polymerase
Adam B Shapiro
Dear Dr. Shapiro,
Thank you so much for sharing this protocol. It is so helpful.
Sincerely
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