Normally mucin polymers are successfully labeled after release from airway goblet cells, but before secretion it remains a great challenge - compact mucin polymer can be tagged with a fluorophore inside the secretory granules, which acts as cargo in this case.
Hello.
It could be helpful if you provide some details about your experiments and approaches. Do you want to see that in vivo, in cell culture, fixed samples, histologycal samples? Can you use transfection with labelled markers?
Hi Patricia, Many thanks for your response.
FYI, It is not a fixed sample or histology experiment rather it is an experiment to look in vivo the cell culture - what is happened to the content of secretory granule/vesicle after loading stimulating reagents on live cells? Idea is, after loading stimulants, the cells try to release secretory granules and granules will burst near the cell membrane, and we would be able to see the behavior of that granule-content, if they are labeled/tagged with an efficient fluorescent marker before release or in other words during synthesis.
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