Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Do you have some articles to recommend about comparative experimental study about gas and water flooding with oil reservoirs?

I would like to know in the oil extraction, which is more efficient: gas flooding or water flooding? Will water flooding be more efficient in hydrophilic reservoirs with capillary...

29 December 2020 3,471 3 View

What does this PZC data mean?

I measured different kinds of catalysts which were dispersed in DI water.I firstly added certain amount of HCl and then used NaOH to titrate and measure the pH value. What does this result mean?

27 December 2020 5,728 4 View

How to inhibit the ion conversion between Mn4 + and MN2 +?

Dear scientists, I have a question to ask you, it is known that Mn4+ is beneficial to the soil, but part of Mn4+ in the soil is easy to be converted to MN2 +, is there any way to inhibit its ion...

21 November 2020 1,200 5 View

Failure in using Gene Pulser Xcell (Biorad) ?

02 October 2020 8,642 1 View

Hi everyone, how can we remove the snow-covered areas in global scale?

We obtained the global results using remote sensing reanalysis dataset. But the snow-coverd areas should be removed (like the blank area in the picture below, sources: Purdy et al., 2016). Does...

20 September 2020 3,463 5 View

How to set the fluent simulation species transport density item?

I use fluent to simulate species transport. The medium is sulfur hexafluoride and air. There is a density item under the mixture, and there is also under the fluid. Is there any difference between...

29 July 2020 7,414 4 View

How to detect brown pigments generated in red wine aging process ?

During aging or storage, the concentration of monomeric anthocyanins decreases rapidly, while low level of polymeric anthocyanins generates, and the color of wine turns brick-red gradually. In...

17 July 2020 6,032 4 View

How to calculate mode overlap ratio in Comsol ?

As is shown in attachment, there is hybrid plasmonic waveguide (HPW) and dielectric waveguide (DW), I want know how to calculate mode overlap ratio to evaluated the mode distribution similarity...

14 July 2020 2,275 3 View

Is there any organic solvent for MnCO3?

Since that my experiment could not be proceeded in aqueous. Anyone who know is there any organic solvent for MnCO3, the concentration could be as low as several mg/mL. Thanks

02 July 2020 9,654 19 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View