I prepared silver nanoparticles and i want to know if they are mono-dispersed
does zetasizer is sufficient?
What pdi value for mono-dispersed Ag nanoparticles?
Thanks Dr John and Dr Alexey.
From you answer i can conclude that TEM more reliable for mondispesity determination , So i will use TEM and check by DLS.
Depends what monodispersity means for you. Looking stringent enough you will not find such in practice. If you want to make sure that there is no oversize fraction in the range of 1 % or less, you will have to either count a very large number by TEM or use a different method. DLS may see only the larger ones. Separating methods like analytical centrifugation may bridge this gap and will enable to detect a small number of larger particles if present.
To study the monodispersity of your NP's I will suggest you to do TEM analysis. Moreover, If the PDI is;
less than 0.1 then it means very good monodisperse NP's
less than 0.2 then it means good monodisperse NP's
less than 0.3 then it means average monodisperse NP's
less than 0.4 then it means normally monodisperse NLP's
0.5 or greater than 0.5 then it means not monodisperse NLP's
In addition to this, I want to clarify you about the size of AgNP's because their size depends on colour variations. And if you are expert in AgNP's then you can assume the size of AgNP's based on their colour. Moreover there are a no. of methods of prep of AgNP's with different sizes. If your prepared AgNP's are not monodispersed means PDI is high then tell me the method by which you are preparing the AgNP's, so i can precisely give the answer relevant to the method. Moreover, keep in mind that it is not compulsory that the size shown by DLS resembles with the size of AgNP's shown by TEM.
Cheers
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